Composite

Part:BBa_K1925006

Designed by: Zi Yue Wang   Group: iGEM16_Fudan   (2016-10-11)


mitronic GFP backbone

To express three shRNAs simultaneously within single transcript, we design artificial introns containing shRNAs within GFP's CDS flanked with engineering restriction enzyme sites. shRNAs could be easily inserted through digestion and ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 448
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


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